Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions

• Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Fluorescence

Growth curve

Protein fusion

Yeast

budding yeast

growth rate

in vivo study

nonhuman

protein analysis

yeast cell

genetics

metabolism

Saccharomyces cerevisiae

coloring agent

photoprotein

Saccharomyces cerevisiae protein

Coloring Agents

Luminescent Proteins

Saccharomyces cerevisiae Proteins

Saccharomycetales

Publikations- och innehållstyp

ref (ämneskategori)

kap (ämneskategori)