Vesicle Collision Protocols for the Study of Quantum Size and Exocytotic Fraction Released

• We review the methods of vesicle impact electrochemical cytometry, intracellular impact electrochemical cytometry, and single cell amperometry and their application to measuring the storage of neurotransmitters in cellular vesicles. We provide protocols to measure vesicle content, the release of catecholamines, and from there the fraction of transmitter released in each exocytosis event. The focus here has been a combination of methods to evaluate factors related to neuronal function at the cellular level and implications in, for example, cognition.

Ämnesord

NATURVETENSKAP  -- Kemi -- Oorganisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Inorganic Chemistry (hsv//eng)

Nyckelord

Electrochemical cytometry

Electrochemistry

Exocytosis

Neurotransmitters

Vesicles

Publikations- och innehållstyp

vet (ämneskategori)

kap (ämneskategori)