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Yeast Mitoribosome ...
Yeast Mitoribosome Purification and Analyses by Sucrose Density Centrifugation and Immunoprecipitation
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- Aufschnaiter, Andreas, Dr. rer. nat. 1988- (författare)
- Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
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- Carlström, Andreas (författare)
- Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
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- Ott, Martin, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden
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(creator_code:org_t)
- Humana Press, 2023
- 2023
- Engelska.
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Ingår i: Methods in Molecular Biology. - : Humana Press. - 1064-3745 .- 1940-6029. ; , s. 119-132, s. 119-132
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Abstract
Ämnesord
Stäng
- Mitochondrial protein biosynthesis is maintained by an interplay between the mitochondrial ribosome (mitoribosome) and a large set of protein interaction partners. This interactome regulates a diverse set of functions, including mitochondrial gene expression, translation, protein quality control, and respiratory chain assembly. Hence, robust methods to biochemically and structurally analyze this molecular machinery are required to understand the sophisticated regulation of mitochondrial protein biosynthesis. In this chapter, we present detailed protocols for immunoprecipitation, sucrose cushions, and linear sucrose gradients to purify and analyze mitoribosomes and their interaction partners.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Immunoprecipitation
- Mitochondria
- Mitoribosome
- Sucrose cushion
- Sucrose gradient
- Yeast
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