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Role of hexose tran...
Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae
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- Elbing, Karin, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Larsson, Christer, 1958 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bill, Roslyn M. (författare)
- Aston University
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- Albers, Eva, 1966 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Snoep, J. L. (författare)
- Universiteit Stellenbosch,Stellenbosch University
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- Boles, E. (författare)
- Johann Wolfgang Goethe Universität Frankfurt am Main,Goethe University Frankfurt
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- Hohmann, Stefan, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
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- Gustafsson, Lena, 1949 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2004
- 2004
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 70:9, s. 5323-5330
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The yeast Saccharomyces cerevisiae predominantly ferments glucose to ethanol at high external glucose concentrations, irrespective of the presence of oxygen. In contrast, at low external glucose concentrations and in the presence of oxygen, as in a glucose-limited chemostat, no ethanol is produced. The importance of the external glucose concentration suggests a central role for the affinity and maximal transport rates of yeast's glucose transporters in the control of ethanol production. Here we present a series of strains producing functional chimeras between the hexose transporters Hxt1 and Hxt7, each of which has distinct glucose transport characteristics. The strains display a range of decreasing glycollytic rates resulting in a proportional decrease in ethanol production. Using these strains, we show for the first time that at high glucose levels, the glucose uptake capacity of wild-type S. cerevisiae does not control glycolytic flux during exponential batch growth. In contrast, our chimeric Hxt transporters control the rate of glycollysis to a high degree. Strains whose glucose uptake is mediated by these chimeric transporters will undoubtedly provide a powerful tool with which to examine in detail the mechanism underlying the switch between fermentation and respiration in S. cerevisiae and will provide new tools for the control of industrial fermentations.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- glucose-transport
- chemostat cultures
- hxt genes
- catabolite repression
- sugar transporters
- yeast-cells
- enzymes
- growth
- overexpression
- metabolism
- yeast-cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Elbing, Karin, 1 ...
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Larsson, Christe ...
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Bill, Roslyn M.
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Albers, Eva, 196 ...
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Snoep, J. L.
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Boles, E.
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Hohmann, Stefan, ...
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Gustafsson, Lena ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Applied and Envi ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola