Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles

• Mature human neutrophils contain small amounts of interleukin-8 [CXC chemokine ligand 8 (CXCL-8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL-8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL-8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL-8 production by lipopolysaccharide. After 8 h of stimulation, CXCL-8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL-8-containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL-8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Biological Markers/metabolism

Calnexin/metabolism

Cells

Cultured

Cytoplasmic Granules/*metabolism/secretion/ultrastructure

Endoplasmic Reticulum/*metabolism/secretion/ultrastructure

Histocytochemistry

Humans

Interleukin-8/immunology/*metabolism/secretion

Intracellular Membranes/drug effects/metabolism/secretion

Lipopolysaccharides

Microscopy

Electron

Transmission

Microscopy

Immunoelectron

Neutrophils/*metabolism/secretion/ultrastructure

Organelles/*metabolism/secretion/ultrastructure

Research Support

Non-U.S. Gov't

Secretory Vesicles/*metabolism/secretion/ultrastructure

Subcellular Fractions

cell activation

subcellular organization

chemokines

inflammation

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)