Single-cell irradiation from [211At] astatine-labeled C215 monoclonal antibody: improved estimates of radiosensitivity from measurements on cellular uptake and retention

• New data on the biological effect of 211At-C215 monoclonal antibody in a slowly rotating, widely dispersed single-cell suspension of the human cancer cell line Colo-205 is presented. Cell growth curves of each experiment were used to calculate an apparent cell survival after irradiation. Uptake measurements provided the data needed to calculate the average number of 211At decays per cell in the cell suspension. The results from each experiment were then fit to a mono-exponential function. From the exponential fit, an average of 35 +/- 2 (SD) astatine-211 decays per cell are required for 37% apparent cell survival (D0).

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

Adenocarcinoma/*pathology

*Alpha Particles

Antibodies

Monoclonal/*metabolism

*Astatine/metabolism

Cell Culture Techniques/methods

Cell Survival/radiation effects

Colonic Neoplasms/*pathology

Dose-Response Relationship

Radiation

Humans

Isotope Labeling/methods

Linear Energy Transfer

Radiation Tolerance

Rotation

Suspensions

Tumor Cells

Cultured/metabolism/radiation effects

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)