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Electron paramagnet...
Electron paramagnetic resonance spectroscopy of site-directed spin labels reveals the structural heterogeneity in the N-terminal domain of apoA-I in solution
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- Lagerstedt, Jens O., 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Wallenberglaboratoriet,Institute of Medicine, Department of Molecular and Clinical Medicine,Wallenberg Laboratory
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Budamagunta, M. S. (författare)
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Oda, M. N. (författare)
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visa fler...
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Voss, J. C. (författare)
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visa färre...
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(creator_code:org_t)
- 2007
- 2007
- Engelska.
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Ingår i: J Biol Chem. - 0021-9258. ; 282:12, s. 9143-9
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Apolipoprotein A-I (apoA-I) is the major protein constituent of high density lipoprotein (HDL) and plays a central role in phospholipid and cholesterol metabolism. This 243-residue long protein is remarkably flexible and assumes numerous lipid-dependent conformations. Consequently, definitive structural determination of lipid-free apoA-I in solution has been difficult. Using electron paramagnetic spectroscopy of site-directed spin labels in the N-terminal domain of apoA-I (residues 1-98) we have mapped a mixture of secondary structural elements, the composition of which is consistent with findings from other in-solution methods. Based on side chain mobility and their accessibility to polar and non-polar spin relaxers, the precise location of secondary elements for amino acids 14-98 was determined for both lipid-free and lipid-bound apoA-I. Based on intermolecular dipolar coupling at positions 26, 44, and 64, these secondary structural elements were arranged into a tertiary fold to generate a structural model for lipid-free apoA-I in solution.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Apolipoprotein A-I
- Crystallization
- Dimerization
- Electron Spin Resonance Spectroscopy/*methods
- Humans
- Lipids/chemistry
- Models
- Molecular
- Molecular Conformation
- Mutagenesis
- Protein Binding
- Protein Conformation
- Protein Folding
- Protein Structure
- Secondary
- Protein Structure
- Tertiary
- *Spin Labels
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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