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Wnt-signaling is ma...
Wnt-signaling is maintained and adipogenesis inhibited by TNFalpha but not MCP-1 and resistin
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- Hammarstedt, Ann, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Isakson, Petter, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Gustafson, Birgit, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Smith, Ulf, 1943 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- Engelska.
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Ingår i: Biochem Biophys Res Commun. - : Elsevier BV. - 0006-291X. ; 357:3, s. 700-6
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Type 2 diabetes and obesity with enlarged fat cells are associated with low-grade systemic inflammation, impaired adipogenesis as well as the recruitment of inflammatory cells into the adipose tissue. Cytokines like TNFalpha and IL-6 are secreted by the inflammatory cells and have been shown to impair normal adipocyte differentiation. An important mechanism whereby these cytokines inhibit adipogenesis is by maintaining an active Wnt-signaling pathway. Also other cytokines like MCP-1 and resistin are involved in the inflammatory process and are secreted by macrophages. If these cytokines also affect Wnt-signaling and adipocyte differentiation is currently unclear. In the present study, we show that while TNFalpha is able to maintain an active Wnt-signaling, induce inflammation and completely block adipose cell differentiation, no effect was found by either MCP-1 or resistin on these processes. Addition of the thiazolidinedione, pioglitazone, was found to antagonize the effect of TNFalpha on the Wnt-signaling process and, consequently, promote adipogenesis.
Nyckelord
- 3T3-L1 Cells
- Adipocytes/cytology/drug effects/metabolism
- Adipogenesis/*drug effects
- Animals
- Blotting
- Western
- Cell Differentiation/drug effects
- Chemokine CCL2/metabolism/pharmacology
- Cyclin D1/genetics
- Gene Expression Regulation/drug effects
- Hypoglycemic Agents/pharmacology
- Indoleacetic Acids/metabolism
- Mice
- RNA
- Messenger/genetics/metabolism
- Resistin/pharmacology
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction/*drug effects/physiology
- Thiazolidinediones/pharmacology
- Time Factors
- Transcription Factors/genetics
- Tumor Necrosis Factor-alpha/*pharmacology
- Wnt Proteins/*physiology
- beta Catenin/metabolism
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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