Oncostatin M signaling in human glioma cell lines.

• We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFRbeta and OSMRbeta. OSM treatment induced phosphorylation of STAT3 and STAT1 indicating presence of a functional JAK/STAT pathway. No OSM effect on proliferation was observed. OSM gave no protective effects against tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced cytotoxicity.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

Apoptosis Regulatory Proteins

Astrocytes

cytology

drug effects

Base Sequence

Cell Line

Tumor

Cell Survival

drug effects

Cells

Cultured

Cytokines

pharmacology

DNA Primers

Glioma

physiopathology

Humans

Membrane Glycoproteins

pharmacology

Oncostatin M

Peptides

genetics

pharmacology

Receptors

Cytokine

genetics

Receptors

Oncostatin M

Reverse Transcriptase Polymerase Chain Reaction

Signal Transduction

drug effects

TNF-Related Apoptosis-Inducing Ligand

Tumor Necrosis Factor-alpha

pharmacology

cytotoxicity

STAT3

proliferation

oncostatin M

astrocytoma

brain tumor

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