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The immunohistochem...
The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity
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- Björk-Eriksson, Thomas, 1960 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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West, C. (författare)
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- Nilsson, A. (författare)
- Karolinska Institutet
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Magnusson, B. (författare)
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Svensson, M. (författare)
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Karlsson, E. (författare)
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Slevin, N. (författare)
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- Lewensohn, R. (författare)
- Karolinska Institutet
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- Mercke, Claes, 1941 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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(creator_code:org_t)
- 1999
- 1999
- Engelska.
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Ingår i: Int J Radiat Oncol Biol Phys. - 0360-3016. ; 45:4, s. 1005-10
- Relaterad länk:
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https://gup.ub.gu.se...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- PURPOSE: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers. METHODS AND MATERIALS: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53. RESULTS: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p<0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17). CONCLUSIONS: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- *Antigens
- Nuclear
- Calcium-Binding Proteins/*metabolism
- *DNA Helicases
- DNA
- Neoplasm/*radiation effects
- DNA-Binding Proteins/*metabolism
- Female
- Head and Neck Neoplasms/genetics/*metabolism/radiotherapy
- Humans
- Immunohistochemistry
- Male
- Neoplasm Proteins/*metabolism
- Nuclear Proteins/*metabolism
- Radiation Tolerance
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Björk-Eriksson, ...
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West, C.
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Nilsson, A.
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Magnusson, B.
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Svensson, M.
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Karlsson, E.
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visa fler...
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Slevin, N.
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Lewensohn, R.
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Mercke, Claes, 1 ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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Int J Radiat Onc ...
- Av lärosätet
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Göteborgs universitet
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Karolinska Institutet