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Impact of natural v...
Impact of natural variation in bacterial F17G adhesins on crystallization behaviour.
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Buts, Lieven (författare)
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Wellens, Adinda (författare)
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Van Molle, Inge (författare)
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Wyns, Lode (författare)
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Loris, Remy (författare)
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- Lahmann, Martina, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden
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Oscarson, Stefan (författare)
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De Greve, Henri (författare)
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Bouckaert, Julie (författare)
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(creator_code:org_t)
- International Union of Crystallography (IUCr), 2005
- 2005
- Engelska.
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Ingår i: Acta crystallographica. Section D, Biological crystallography. - : International Union of Crystallography (IUCr). - 0907-4449. ; 61:8, s. 1149-1159
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Since the introduction of structural genomics, the protein has been recognized as the most important variable in crystallization. Recent strategies to modify a protein to improve crystal quality have included rationally engineered point mutations, truncations, deletions and fusions. Five naturally occurring variants, differing in 1-18 amino acids, of the 177-residue lectin domain of the F17G fimbrial adhesin were expressed and purified in identical ways. For four out of the five variants crystals were obtained, mostly in non-isomorphous space groups, with diffraction limits ranging between 2.4 and 1.1 A resolution. A comparative analysis of the crystal-packing contacts revealed that the variable amino acids are often involved in lattice contacts and a single amino-acid substitution can suffice to radically change crystal packing. A statistical approach proved reliable to estimate the compatibilities of the variant sequences with the observed crystal forms. In conclusion, natural variation, universally present within prokaryotic species, is a valuable genetic resource that can be favourably employed to enhance the crystallization success rate with considerably less effort than other strategies.
Ämnesord
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Adhesins
- Bacterial
- chemistry
- genetics
- Amino Acid Sequence
- Crystallization
- methods
- Crystallography
- X-Ray
- Disaccharides
- chemistry
- Escherichia coli Proteins
- chemistry
- genetics
- Models
- Molecular
- Molecular Sequence Data
- Protein Structure
- Tertiary
- Sequence Alignment
- Variation (Genetics)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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