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Impaired regulatory...
Impaired regulatory T cell function in germ-free mice.
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- Östman, Sofia M, 1974 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Rask, Carola, 1970 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Wold, Agnes E, 1955 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Hultkrantz, Susanne, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Telemo, Esbjörn, 1953 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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(creator_code:org_t)
- Wiley, 2006
- 2006
- Engelska.
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Ingår i: European journal of immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 36:9, s. 2336-46
- Relaterad länk:
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https://onlinelibrar...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Regulatory T cells (Treg) are crucial for the maintenance of tolerance to auto-antigens and harmless exogenous antigens. Here, we studied the role of the commensal microbiota for the development and function of Treg. CD4+CD25+ T cells were obtained from peripheral lymph nodes (PLN) and mesenteric lymph nodes (MLN) of germ-free (GF) and conventional (conv) NMRI mice and tested for phenotype and functional suppressive capacity. CD4+CD25+ T cells from GF mice showed a lower relative gene expression of fork head box p3 gene (Foxp3) and were not as potent suppressors in vitro as CD4+CD25+ T cells from conv animals. Intracellular staining for Foxp3 and CTLA-4 revealed proportional and regional differences in putative Treg subsets between conv and GF mice. Fewer of the CD4+CD25+ T cells in GF MLN expressed Foxp3 and CTLA-4, while the expression of these markers was similar amongst the CD4+CD25+ T cells in PLN of conv and GF mice. The largest difference between conv and GF Treg was observed in the liver draining celiac lymph node, where GF mice had fewer putative Treg as compared to conv mice. We propose that the presence of a microbial flora favors the development of a fully functional Treg population.
Nyckelord
- Animals
- Antigens
- CD
- Antigens
- CD4
- biosynthesis
- immunology
- Antigens
- Differentiation
- biosynthesis
- immunology
- Flow Cytometry
- Forkhead Transcription Factors
- biosynthesis
- immunology
- Gene Expression
- Germ-Free Life
- Immune Tolerance
- Interleukin-10
- biosynthesis
- Interleukin-13
- biosynthesis
- Lymph Nodes
- cytology
- immunology
- Mice
- Phenotype
- RNA
- Messenger
- analysis
- Receptors
- Interleukin-2
- biosynthesis
- immunology
- T-Lymphocyte Subsets
- immunology
- T-Lymphocytes
- Regulatory
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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