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Use of biliary PAH ...
Use of biliary PAH metabolites as a biomarker of pollution in fish from the Baltic Sea
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Vuorinen, P. J. (author)
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Keinänen, M. (author)
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Vuontisjärvi, H (author)
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Barsiene, J. (author)
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Broeg, K. (author)
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- Förlin, Lars, 1950 (author)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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Gercken, J. (author)
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Kopeca, J (author)
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Köhler, A. (author)
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- Parkkonen, Jari, 1959 (author)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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Pempkowiak, J. (author)
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Schiedek, D. (author)
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: Marine Pollution Bulletin. - : Elsevier BV. - 0025-326X. ; 53:8-9, s. 479-487
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- During field campaigns of the BEEP project (Biological Effects of Environmental Pollution in Marine Coastal Ecosystems) in 2001–2002, metabolites of polycyclic aromatic hydrocarbons (PAHs) were determined in bile samples from three fish species, flounder (Platichthys flesus), perch (Perca fluviatilis) and eelpout (Zoarces viviparus), from four separate areas in the Baltic Sea. Two determination methods were applied: fixed wavelength fluorescence (FF) for pyrene-type metabolites and high-performance liquid chromatography with fluorescence detection (HPLC). There was a good correlation between the FF method and 1-OH pyrene determined by HPLC. Normalisation of the FF data for absorbance at 380 nm or bile protein concentrations greatly increased variance in one third and decreased it in two thirds of the cases and resulted in a loss of significant differences (protein normalisation) between the sampling stations, but normalisation of the HPLC data had little effect on the results. The biliary PAH metabolite content was usually higher in males than in females. In perch and eelpout the biliary PAH contents were at similar levels, whereas in flounder the levels were lower. The sampling areas arranged in decreasing order of biliary PAH contents were: Wismar Bay > Gulf of Gdansk > Lithuanian coast > Kvadofjärden (reference area). It is concluded that FF with un-normalised data is a reliable and simple method for monitoring purposes and only one sex of a selected species should be used.
Subject headings
- NATURVETENSKAP -- Biologi -- Zoologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Zoology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Polycyclic aromatic hydrocarbon
- PAH
- FAC
- Baltic Sea
- Flounder
- Perch
- Eelpout
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Vuorinen, P. J.
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Keinänen, M.
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Vuontisjärvi, H
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Barsiene, J.
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Broeg, K.
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Förlin, Lars, 19 ...
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show more...
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Gercken, J.
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Kopeca, J
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Köhler, A.
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Parkkonen, Jari, ...
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Pempkowiak, J.
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Schiedek, D.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Zoology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Marine Pollution ...
- By the university
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University of Gothenburg