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Parathyroid Ca(2+)-...
Parathyroid Ca(2+)-conducting currents are modulated by muscarinic receptor agonists and antagonists.
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Chang, W (författare)
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Chen, T H (författare)
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Pratt, S A (författare)
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visa fler...
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Yen, B (författare)
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- Fu, Michael, 1963 (författare)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Wallenberg Laboratory
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Shoback, D (författare)
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visa färre...
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(creator_code:org_t)
- 1997
- 1997
- Engelska.
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Ingår i: The American journal of physiology. - 0002-9513. ; 273:5 Pt 1
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Parathyroid cells express Ca(2+)-conducting cation currents, which are activated by raising the extracellular Ca2+ concentration ([Ca2+]o) and blocked by dihydropyridines. We found that acetylcholine (ACh) inhibited these currents in a reversible, dose-dependent manner (50% inhibitory concentration approximately equal to 10(-8) M). The inhibitory effects could be mimicked by the agonist (+)-muscarine. The effects of ACh were blunted by the antagonist atropine and reversed by removing ATP from the pipette solution (+)-Muscarine enhanced the adenosine 3',5'-cyclic monophosphate (cAMP) production by 30% but had no effect on inositol phosphate accumulation in parathyroid cells. Oligonucleotide primers, based on sequences of known muscarinic receptors (M1-M5), were used in reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify receptor cDNA from parathyroid poly (A)+ RNA. RT-PCR products displayed > 90% nucleotide sequence identity to human M2- and M4-receptor cDNAs. Expression of M2-receptor protein was further confirmed by immunoblotting and immunocytochemistry. Thus parathyroid cells express muscarinic receptors of M2 and possibly M4 subtypes. These receptors may couple to dihydropyridine-sensitive, cation-selective currents through the activation of adenylate cyclase and ATP-dependent pathways in these cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Acetylcholine
- pharmacology
- Amino Acid Sequence
- Animals
- Atropine
- pharmacology
- Base Sequence
- Calcium Channels
- biosynthesis
- drug effects
- physiology
- Cattle
- Cells
- Cultured
- Cyclic AMP
- metabolism
- DNA Primers
- Humans
- Inositol Phosphates
- metabolism
- Kinetics
- Membrane Potentials
- drug effects
- physiology
- Molecular Sequence Data
- Muscarine
- pharmacology
- Muscarinic Agonists
- pharmacology
- Muscarinic Antagonists
- pharmacology
- Nifedipine
- pharmacology
- Parathyroid Glands
- drug effects
- physiology
- Polymerase Chain Reaction
- Receptors
- Muscarinic
- biosynthesis
- genetics
- Sequence Alignment
- Sequence Homology
- Amino Acid
- Sequence Homology
- Nucleic Acid
- Tetraethylammonium
- pharmacology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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