Immunocytochemical localization of M2 muscarinic receptors in rat ventricles with anti-peptide antibodies.

• We produced antibodies against a synthetic peptide corresponding to amino acids 168-192 of the second extracellular loop of the M2 human muscarinic receptor in rabbits. In immunoblot, affinity-purified antibodies specifically recognized a major band of rat ventricular muscarinic receptor protein with a molecular weight of about 80 KD. This recognition could be blocked by pre-incubation with peptide. Moreover, with both light (LM) and electron microscopic (EM) immunocytochemistry techniques, muscarinic receptors were detected on sarcolemma and T-tubules of rat cardiomyocytes. In addition, immunoreactions were localized in membranes of capillaries. Likewise, these reactivities were abolished by pre-incubation with peptide. These results suggest that the antibodies against the second extracellular loop of human M2 muscarinic receptor could specifically recognize rat ventricular muscarinic receptor protein and could be a powerful tool to study the fate of this receptor under different pathological or physiological conditions.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Acetylcholine

chemistry

Amino Acid Sequence

Animals

Antibodies

Fluorescent Antibody Technique

Heart Ventricles

chemistry

ultrastructure

Humans

Immunoblotting

Immunohistochemistry

Microscopy

Immunoelectron

methods

Molecular Sequence Data

Peptides

Rabbits

Rats

Receptors

Muscarinic

analysis

chemistry

immunology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)