Microarray analysis of blood microvessels from PDGF-B and PDGF-Rbeta mutant mice identifies novel markers for brain pericytes.

• Normal blood microvessels are lined by pericytes, which contribute to microvessel development and stability through mechanisms that are poorly understood. Pericyte deficiency has been implicated in the pathogenesis of microvascular abnormalities associated with diabetes and tumors. However, the unambiguous identification of pericytes is still a problem because of cellular heterogeneity and few available molecular markers. Here we describe an approach to identify pericyte markers based on transcription profiling of pericyte-deficient brain microvessels isolated from platelet-derived growth factor (PDGF-B)-/- and PDGF beta receptor (PDGFRbeta)-/- mouse mutants. The approach was validated by the identification of known pericyte markers among the most down-regulated genes in PDGF-B-/- and PDGFRbeta-/- microvessels. Of candidates for novel pericyte markers, we selected ATP-sensitive potassium-channel Kir6.1 (also known as Kcnj8) and sulfonylurea receptor 2, (SUR2, also known as Abcc9), both part of the same channel complex, as well as delta homologue 1 (DLK1) for in situ hybridization, which demonstrated their specific expression in brain pericytes of mouse embryos. We also show that Kir6.1 is highly expressed in pericytes in brain but undetectable in pericytes in skin and heart. The three new brain pericyte markers are signaling molecules implicated in ion transport and intercellular signaling, potentially opening new windows on pericyte function in brain microvessels.

Nyckelord

ATP-Binding Cassette Transporters

analysis

Animals

Biological Markers

analysis

Brain

blood supply

cytology

Capillaries

cytology

Embryo

Gene Expression Profiling

Intercellular Signaling Peptides and Proteins

Membrane Proteins

analysis

Mice

Mice

Mutant Strains

Microarray Analysis

methods

Pericytes

chemistry

Potassium Channels

Inwardly Rectifying

analysis

Proto-Oncogene Proteins c-sis

deficiency

Receptor

Platelet-Derived Growth Factor beta

deficiency

Repressor Proteins

analysis

Tissue Distribution

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)