Abnormal reactivity of muller cells after retinal detachment in mice deficient in GFAP and vimentin.

• PURPOSE: To determine the roles of glial fibrillary acidic protein (GFAP) and vimentin in M?ller cell reactivity. METHODS: Retinal detachments were created in mice deficient for GFAP and vimentin (GFAP(-/-)vim(-/-)) and age-matched wild-type (wt) mice. The reactivity of the retina was studied by immunofluorescence and electron microscopy. RESULTS: M?ller cell morphology was different and glutamine synthetase immunoreactivity was reduced in the undisturbed GFAP(-/-)vim(-/-) retinas. After retinal detachment, M?ller cells formed subretinal glial scars in the wt mice. In contrast, such scars were not observed in GFAP(-/-)vim(-/-) mice. M?ller cells, which normally elongate and thicken in response to detachment, appeared compressed, thin, and "spikey" in the GFAP(-/-)vim(-/-) mice. The end foot region of M?ller cells in the GFAP(-/-)vim(-/-) mice often sheared away from the rest of the retina during detachment, corroborating earlier results showing decreased resistance of this region in GFAP(-/-)vim(-/-) retinas to mechanical stress. In regions with end foot shearing, ganglion cells showed intense neurite sprouting, as revealed by anti-neurofilament labeling, a response rarely observed in wt mice. CONCLUSIONS: M?ller cells are subtly different in the GFAP(-/-)vim(-/-) mouse retina before detachment. The end foot region of these cells may be structurally reinforced by the presence of the intermediate filament cytoskeleton, and our data suggest a critical role for these proteins in M?ller cell reaction to retinal detachment and participation in subretinal gliosis.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Neurosciences (hsv//eng)

Nyckelord

Animals

Disease Models

Animal

Fluorescent Antibody Technique

Indirect

Glutamate-Ammonia Ligase

metabolism

Male

Mice

Mice

Inbred C57BL

Mice

Knockout

Microscopy

Confocal

Microscopy

Electron

Transmission

Nerve Tissue Proteins

physiology

Neuroglia

metabolism

pathology

Photoreceptor Cells

Vertebrate

metabolism

pathology

Retina

metabolism

pathology

Retinal Bipolar Cells

metabolism

pathology

Retinal Detachment

metabolism

pathology

Retinal Ganglion Cells

metabolism

pathology

Retinal Horizontal Cells

metabolism

pathology

Rod Opsins

metabolism

S100 Proteins

metabolism

Up-Regulation

Vimentin

physiology

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