SGD1 encodes an essential nuclear protein of Saccharomyces cerevisiae that affects expression of the GPD1 gene for glycerol 3-phosphate dehydrogenase.

• We here report the identification of the previously uncharacterized SGD1 gene, encoding a 102.8-kDa protein containing a leucine zipper region and a bipartite nuclear localization signal. Deletion of SGD1 results in loss of cell viability, while an increased dosage of SGD1 partially suppresses the osmosensitivity of pbs2 delta and hog1 delta mutants that are defective in the osmosignaling high osmolarity glycerol (HOG) mitogen-activated protein kinase pathway. The rescued mutants display a partially re-established transcriptional control of the osmostress-induced expression of GPD1, a target gene of the HOG pathway encoding NAD(+)-dependent glycerol 3-phosphate dehydrogenase, and a partially recovered hyperosmolarity-induced production of glycerol. Consistent with Sgd1p affecting the transcriptional control of GPD1, a functional green fluorescent protein tagged Sgd1p is localized to the cell nucleus.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Cell Division

genetics

Cloning

Molecular

Fluorescent Antibody Technique

Indirect

Gene Deletion

Gene Expression Regulation

Enzymologic

Gene Expression Regulation

Fungal

Genes

Essential

genetics

Glycerol

metabolism

Glycerolphosphate Dehydrogenase

genetics

Green Fluorescent Proteins

Luminescent Proteins

genetics

metabolism

Microscopy

Fluorescence

Mutation

Nuclear Proteins

genetics

metabolism

Osmolar Concentration

Recombinant Fusion Proteins

genetics

metabolism

Saccharomyces cerevisiae

genetics

growth & development

isolation & purification

Saccharomyces cerevisiae Proteins

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)