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Cytolethal distendi...
Cytolethal distending toxin upregulates RANKL expression in Jurkat T-cells.
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Belibasakis, Georgios N (författare)
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- Brage, Monica, 1964- (författare)
- Umeå universitet,Parodontologi,Patologi,Aa-gruppen
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- Lagergård, Teresa, 1946 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Johansson, Anders, 1955- (författare)
- Umeå universitet,Institutionen för odontologi
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Belibasakis, George, 1977- (författare)
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(creator_code:org_t)
- Copenhagen : Wiley, 2008
- 2008
- Engelska.
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Ingår i: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. - Copenhagen : Wiley. - 0903-4641 .- 1600-0463. ; 116:6, s. 499-506
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Cytolethal distending toxin, a bacterial exotoxin produced by a number of Gram-negative species, causes growth arrest and morphological alterations in host cells. Among these species are Haemophilus ducreyi, the etiological agent of chancroid, and the periodontal pathogen Aggregatibacter actinomycetemcomitans, highly implicated in localized aggressive periodontitis. CDT induces receptor activator of NF-kappaB ligand (RANKL) expression in periodontal fibroblasts, the key bone-resorbing cytokine. T-cells are actively involved in localized inflammation-induced bone destruction, including periodontitis. The aim of this study was to investigate the effects of purified CDT on the expression of RANKL and its decoy receptor osteoprotegerin (OPG), in the Jurkat T-cell line. Quantitative real-time PCR indicated that 100 pg/ml of purified H. ducreyi CDT upregulated RANKL mRNA expression by 2.2-fold, after 24 h of exposure. This increase was corroborated by a 2.0-fold increase in RANKL protein release, as determined by ELISA. OPG was not detected in this experimental system. In conclusion, CDT enhances RANKL expression in T-cells, denoting that these cells are a potential target for the toxin and strengthening the potential link between this virulence factor and mechanisms associated with localized bone resorption.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- Bacterial Toxins
- isolation & purification
- metabolism
- toxicity
- Cell Cycle
- Cell Line
- Tumor
- Haemophilus ducreyi
- Humans
- Jurkat Cells
- NF-kappa B
- metabolism
- Osteoprotegerin
- metabolism
- RANK Ligand
- metabolism
- T-Lymphocytes
- metabolism
- Cdt
- Oral microbiology
- mikrobiologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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