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Osteoprotegerin mRN...
Osteoprotegerin mRNA is increased by interleukin-1 alpha in the human osteosarcoma cell line MG-63 and in human osteoblast-like cells.
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- Vidal, Olle (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Sjögren, Klara, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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Eriksson, B I (author)
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Ljunggren, O (author)
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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(creator_code:org_t)
- Elsevier BV, 1998
- 1998
- English.
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In: Biochemical and biophysical research communications. - : Elsevier BV. - 0006-291X. ; 248:3, s. 696-700
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https://doi.org/10.1...
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Abstract
Subject headings
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- Osteoprotegerin (OPG) is a soluble receptor for the Osteoprotegerin-Ligand (OPGL) which is expressed on osteoblasts and mediates the signal for osteoclast differentiation. In the present study we demonstrate that OPG mRNA levels in MG-63 cells are increased in a dose-dependent manner after 8 h of treatment with IL-1 alpha (338 +/- 53% over control at 25 U/ml). Interleukin-6 (IL-6), under similar culture conditions, does not affect OPG mRNA levels. Time-course studies show that IL-1 alpha (25 U/ml) causes a transient increase of OPG mRNA levels in MG-63 cells, peaking after 4 h of treatment. An increase of the OPG transcript occurs in hOB cells at 0.5 h which is still present after 24 h of IL-1 alpha treatment. In MG-63 cells neither basal-nor IL-1 alpha-induced OPG mRNA levels are altered by the translational inhibitor cycloheximide. These results suggest that expression of OPG in osteoblasts may be regulated by IL-1 alpha.
Keyword
- Bone Neoplasms
- Cell Differentiation
- Cycloheximide
- pharmacology
- Dose-Response Relationship
- Drug
- Gene Expression Regulation
- drug effects
- Gene Expression Regulation
- Neoplastic
- drug effects
- Glycoproteins
- biosynthesis
- Humans
- Interleukin-1
- pharmacology
- Kinetics
- Osteoblasts
- drug effects
- metabolism
- Osteoclasts
- cytology
- Osteoprotegerin
- Osteosarcoma
- Polymerase Chain Reaction
- RNA
- Messenger
- biosynthesis
- Receptors
- Cytoplasmic and Nuclear
- Receptors
- Tumor Necrosis Factor
- biosynthesis
- Signal Transduction
- Transcription
- Genetic
- drug effects
- Tumor Cells
- Cultured
Publication and Content Type
- ref (subject category)
- art (subject category)
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