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Establishment of a ...
Establishment of a growth hormone responsive chondrogenic cell line from fetal rat tibia.
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- Ohlsson, Claes, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Nilsson, Anders, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för ortopedi,Institute of Surgical Sciences, Department of Orthopaedics
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- Swolin-Eide, Diana, 1968 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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visa fler...
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- Isaksson, Olle, 1943 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Lindahl, Anders, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk kemi/transfusionsmedicin,Institute of Laboratory Medicine, Dept of Clinical Chemistry/Transfusion Medicine
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visa färre...
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(creator_code:org_t)
- 1993
- 1993
- Engelska.
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Ingår i: Molecular and cellular endocrinology. - 0303-7207. ; 91:1-2, s. 167-75
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Reproducible effects of growth hormone (GH) on primary isolated cells in monolayer are highly dependent on the culture conditions and/or the fraction of GH responsive cells. To study the effect of GH at the cellular level, a homogenous cell line with both GH responsiveness and chondrogenic properties was established. Primary isolated cells from 18-day-old fetal rat tibia were subcultured using a strict protocol for passages (every third day and a seeding density of 15,000/cm2). Of six established cell lines, one fetal tibia cell line No. 5 (FTC 5) expressed adipogenic and chondrogenic properties at a low frequency. Cells from FTC 5 were subcultured in soft agar suspension with the addition of bovine GH (100 ng/ml). After 14 days in culture eight monoclonal cell lines were established from individual large colonies. Two subclones, FTC 5:3 and FTC 5:6, expressed a chondrogenic phenotype as demonstrated by chondrocyte foci, alcian blue staining and production of type II collagen. Further characterization of FTC 5:3 revealed specific binding of bovine GH with an affinity of 1.7 x 10(9) M-1, and approximately 7300 receptors/cell. Northern blot analysis of FTC 5:3 with a 32P-labeled RNA probe complementary to an extracellular part of the rat GH receptor, revealed two major labeled bands (4.0 and 1.2 kilobases). Both GH and insulin-like growth factor-I (IGF-I) stimulated 3H-thymidine uptake in FTC 5:3 (194 +/- 28% and 405 +/- 127% over control, respectively), while proteoglycan synthesis, as measured by [35S]sulphate uptake, was stimulated by IGF-I only (101 +/- 18% over control).(ABSTRACT TRUNCATED AT 250 WORDS)
Nyckelord
- Animals
- Blotting
- Northern
- Cartilage
- cytology
- drug effects
- embryology
- Cell Division
- drug effects
- Clone Cells
- Collagen
- metabolism
- DNA
- biosynthesis
- Growth Hormone
- metabolism
- pharmacology
- Immunohistochemistry
- Insulin-Like Growth Factor I
- pharmacology
- Karyotyping
- Phenotype
- RNA
- analysis
- Rats
- Rats
- Sprague-Dawley
- Receptors
- Somatotropin
- genetics
- metabolism
- Tibia
- embryology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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