Tau in cerebrospinal fluid: a sensitive sandwich enzyme-linked immunosorbent assay using tyramide signal amplification.

• Tau in cerebrospinal fluid (CSF) has been proposed as a diagnostic marker for Alzheimer's disease (AD). This paper presents a new sensitive sandwich ELISA allowing quantitation of tau from 8 microl CSF/well. A human specific monoclonal tau antibody HT7 was used as a capture antibody and a mixture of polyclonal tau antibodies, 92e and R134d was used as reporter antibodies. Tyramide signal amplification (TSA) technology was used in the last step to increase the sensitivity. With this TSA-ELISA, the lowest detection limit for tau was 14.3 pg/ml. Tau levels in CSF were found to be increased in AD patients (807+/-304 pg/ml, p<0.001) compared with controls (252+/-94 pg/ml). Thirty-five of 38 AD cases (92% sensitivity) yielded signals greater than cutoff, while only 1 of 38 control cases (97% specificity) was greater. A highly significant correlation was found between this assay and a commonly used kit, INNOTEST hTAU Antigen.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Psykiatri (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Psychiatry (hsv//eng)

Keyword

Aged

Aged

80 and over

Alzheimer Disease

cerebrospinal fluid

diagnosis

Antibodies

diagnostic use

Biological Markers

analysis

cerebrospinal fluid

Biotin

analogs & derivatives

diagnostic use

Brain

metabolism

physiopathology

Brain Chemistry

physiology

Cerebrospinal Fluid Proteins

analysis

chemistry

metabolism

Enzyme-Linked Immunosorbent Assay

methods

Female

Humans

Indicators and Reagents

Male

Predictive Value of Tests

Reproducibility of Results

Tyramine

analogs & derivatives

diagnostic use

Up-Regulation

physiology

tau Proteins

analysis

cerebrospinal fluid

chemistry

Publication and Content Type

ref (subject category)

art (subject category)