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Antibody Developmen...
Antibody Development - Examples from viral and proteomic studies
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- Carlsson, Fredrika (författare)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- ISBN 9789174732764
- 2012
- Engelska.
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- This thesis, which is based on four original papers, covers different aspects of antibody development. The overall goal of my work has been to obtain an increased understanding of antibody repertoires as they develop in vivo and in vitro. In Paper I-III antibody repertoires was evaluated and used to dissect virus-induced antibody responses and characterize such antibodies. Furthermore, tools for more efficient studies of such characterized antibodies were developed. The study of antibody repertoires in this thesis reveals, not only insights of the composition of antibodies induced by a common virus, but also features for such binders developed by combinatorial and non-combinatorial approaches. Such developed antibodies may contribute with data important for future design of vaccine candidates. In Paper IV, limitations in biomarker verification processes are addressed. A proof-of-concept study was developed, describing an approach with high-throughput potential relying on enrichment of proteins by antibody fragments in complex serum samples followed by an MRM mass spectrometry readout. Taken together, the in vitro developed binders in combination with MRM readout provides a powerful method to quantify protein biomarkers in a high-throughput manner approaching the criteria for clinical usage.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- proteome specific antibodies
- cytomegalovirus
- phage display
- antibody characterization
- antibody development
- antibody repertoires
Publikations- och innehållstyp
- dok (ämneskategori)
- vet (ämneskategori)
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