Sökning: id:"swepub:oai:lup.lub.lu.se:10da6e69-ddfb-4192-991a-e465a325a493" >
Rapid Detection of ...
Rapid Detection of Multiple Classes of β-Lactam Antibiotics in Blood Using an NDM-1 Biosensing Assay
-
- Meng, Qinglai (författare)
- Shanxi University
-
- Wang, Yao (författare)
- Shanxi University
-
- Long, Yali (författare)
- Shanxi University
-
visa fler...
-
- Yue, Aiping (författare)
- Omik Bioscience AB
-
- Mecklenburg, Michael (författare)
- Omik Bioscience AB
-
- Tian, Shuaiyan (författare)
- Shanxi University
-
- Fu, Yujia (författare)
- Shanxi University
-
- Yao, Xiangyu (författare)
- Shanxi University
-
- Liu, Jianyi (författare)
- National Institute of Metrology China
-
- Song, Dewei (författare)
- National Institute of Metrology China
-
- Wu, Changxin (författare)
- Shanxi University
-
- Xie, Bin (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
visa färre...
-
(creator_code:org_t)
- 2021-09-14
- 2021
- Engelska.
-
Ingår i: Antibiotics. - : MDPI AG. - 2079-6382. ; 10:9
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://www.mdpi.com...
-
https://lup.lub.lu.s...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Currently, assays for rapid therapeutic drug monitoring (TDM) of β-lactam antibiotics in blood, which might be of benefit in optimizing doses for treatment of critically ill patients, remain challenging. Previously, we developed an assay for determining the penicillin-class antibiotics in blood using a thermometric penicillinase biosensor. The assay eliminates sample pretreatment, which makes it possible to perform semicontinuous penicillin determinations in blood. However, penicillinase has a narrow substrate specificity, which makes it unsuitable for detecting other classes of β-lactam antibiotics, such as cephalosporins and carbapenems. In order to assay these classes of clinically useful antibiotics, a novel biosensor was developed using New Delhi metallo-β-lactamase-1 (NDM-1) as the biological recognition layer. NDM-1 has a broad specificity range and is capable of hydrolyzing all classes of β-lactam antibiotics in high efficacy with the exception of monobactams. In this study, we demonstrated that the NDM-1 biosensor was able to quantify multiple classes of β-lactam antibiotics in blood plasma at concentrations ranging from 6.25 mg/L or 12.5 mg/L to 200 mg/L, which covered the therapeutic concentration windows of the tested antibiotics used to treat critically ill patients. The detection of ceftazidime and meropenem was not affected by the presence of the β-lactamase inhibitors avibactam and vaborbactam, respectively. Furthermore, both free and protein-bound β-lactams present in the antibiotic-spiked plasma samples were detected by the NDM-1 biosensor. These results indicated that the NDM-1 biosensor is a promising technique for rapid TDM of total β-lactam antibiotics present in the blood of critically ill patients.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Meng, Qinglai
-
Wang, Yao
-
Long, Yali
-
Yue, Aiping
-
Mecklenburg, Mic ...
-
Tian, Shuaiyan
-
visa fler...
-
Fu, Yujia
-
Yao, Xiangyu
-
Liu, Jianyi
-
Song, Dewei
-
Wu, Changxin
-
Xie, Bin
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
- Artiklar i publikationen
-
Antibiotics
- Av lärosätet
-
Lunds universitet