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Axonal outgrowth in...
Axonal outgrowth in muscle grafts made acellular by chemical extraction
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- Arai, Takeru (författare)
- Keio University
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- Kanje, Martin (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Functional zoology,Department of Biology,Faculty of Science
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- Lundborg, Göran (författare)
- Skåne University Hospital
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- Sondell, Mariann (författare)
- Skåne University Hospital
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- Liu, Xiao Lin (författare)
- Skåne University Hospital
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- Dahlin, Lars B. (författare)
- Skåne University Hospital
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(creator_code:org_t)
- 2000
- 2000
- Engelska.
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Ingår i: Restorative Neurology and Neuroscience. - 0922-6028. ; 17:4, s. 165-174
- Relaterad länk:
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Abstract
Ämnesord
Stäng
- Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of basal laminae of the muscle fibers. The freeze/thawed grafts were brittle and lacked elasticity, but resulted in minor changes in major proteins. The axons regenerated through both types of grafts (initial delay 6 days and rate 0.7-0.8 mm/day), which shrunk in length by 25 %. There were no apparent differences with respect to Schwann cells and macrophages. Conclusions: The results suggest that detergent extracted mucle tissue, in which some basal lamina proteins remain but cells are removed, could present a new favourable option for nerve grafting.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Ortopedi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Orthopaedics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Axons
- Extraction
- Macrophages
- Muscle
- Nerve grafts
- Nerve regeneration
- Schwann cells
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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