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Naturally occurring...
Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans
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- Sunds, Anne Vuholm (författare)
- Aarhus University
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- Roland, Ida Schwartz (författare)
- Aarhus University
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- Sundekilde, Ulrik Kræmer (författare)
- Aarhus University
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- Thesbjerg, Martin Nørmark (författare)
- Aarhus University
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- Robinson, Randall (författare)
- University of California, Davis
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Bunyatratchata, Apichaya (författare)
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- Glantz, Maria (författare)
- Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Paulsson, Marie (författare)
- Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Leskauskaite, Daiva (författare)
- Kaunas University of Technology
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- Pihlanto, Anne (författare)
- Natural Resources Institute Finland (Luke)
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- Inglingstad, Ragnhild (författare)
- Norwegian University of Life Sciences
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- Devold, Tove Gulbrandsen (författare)
- Norwegian University of Life Sciences
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- Vegarud, Gerd Elisabeth (författare)
- Norwegian University of Life Sciences
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- Birgisdottir, Bryndis Eva (författare)
- University of Iceland
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- Gudjonsdottir, Maria (författare)
- University of Iceland
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- Barile, Daniela (författare)
- University of California, Davis
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- Larsen, Lotte Bach (författare)
- Aarhus University
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- Poulsen, Nina Aagaard (författare)
- Aarhus University
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(creator_code:org_t)
- 2021-09-28
- 2021
- Engelska.
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Ingår i: Metabolites. - : MDPI AG. - 2218-1989. ; 11:10
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://www.mdpi.com...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value < 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.
Ämnesord
- LANTBRUKSVETENSKAPER -- Husdjursvetenskap -- Husdjursvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Animal and Dairy Sience -- Animal and Dairy Science. (hsv//eng)
Nyckelord
- Cattle breeds
- Mass spectrometry
- Natural glycosidases
- NMR spectroscopy
- O-linked glycans
- Oligosaccharides
- Principal component analysis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Sunds, Anne Vuho ...
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Roland, Ida Schw ...
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Sundekilde, Ulri ...
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Thesbjerg, Marti ...
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Robinson, Randal ...
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Glantz, Maria
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Paulsson, Marie
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Leskauskaite, Da ...
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Pihlanto, Anne
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Inglingstad, Rag ...
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Devold, Tove Gul ...
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Vegarud, Gerd El ...
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Birgisdottir, Br ...
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Gudjonsdottir, M ...
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Barile, Daniela
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Larsen, Lotte Ba ...
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Poulsen, Nina Aa ...
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