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Identification and use of personalized genomic markers for monitoring circulating tumor DNA
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- Chen, Yilun (författare)
- Lund University,Lunds universitet,Translational Oncogenomics,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- George, Anthony M. (författare)
- Lund University,Lunds universitet,Translational Oncogenomics,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Olsson, Eleonor (författare)
- Lund University,Lunds universitet,Translational Oncogenomics,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Saal, Lao H. (författare)
- Lund University,Lunds universitet,Translational Oncogenomics,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2018-05-02
- 2018
- Engelska 20 s.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1064-3745. ; 1768, s. 303-322
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Digital PCR techniques are ideally suited for accurately quantifying trace amounts of target DNA sequences, such as tumor-derived mutant DNA that is present in the blood circulation of patients with cancer. Here, we describe an approach marrying low-coverage whole-genome sequencing of tumor tissues, to enumerate chromosomal rearrangement breakpoints, together with droplet digital PCR (ddPCR)-based personalized rearrangement assays to cost-effectively monitor circulating tumor DNA levels at multiple time-points during the clinical course. The method is generally applicable to essentially any cancer patient, as all cancers harbor unstable genomes, and may have uses for measuring minimal residual disease, response to therapy, and early detection of metastasis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- Cell-free circulating tumor DNA
- Droplet digital PCR
- Liquid biopsy
- Noninvasive diagnosis
- Personalized medicine
- Whole-genome sequencing
Publikations- och innehållstyp
- kap (ämneskategori)
- ref (ämneskategori)
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