Gold-coated capillary based 2,4-dichlorophenoxyacetic acid chemi-lumincscent assays: possibilities towards multianalysis

• The application of gold-coated glass capillaries for the design of a sensitive chemiluminescent immunoassay for 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The gold coating on the glass capillaries has been partially characterized and its effect on enhancing the signal intensity has been measured. A simple photo-multiplier tube-based photon detector is used for this purpose. At least three times improvement in the signal intensity is observed compared to uncoated glass capillaries, with a consequent improvement in the sensitivity of detection. Using such gold-coated glass capillaries, 2,4-D in the range 10(-9) to 10(-13) mol/l is detectable at a precision of +/-15% (CV%) and a limit of detection of 10(-15) mol/l is achievable. The possibility of using such gold-coated capillaries with a portable multianalytical set-up for field studies is also demonstrated.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

pesticides

detection limit

portable multianalytical set-up

precision

capillaries

Au-coated glass capillaries

Au

acid

sensitivity

photo-multiplier tube-based photon detector

signal intensity

glass capillaries

Au coating

sensitive chemiluminescent immunoassay

design

4-dichlorophenoxyacetic acid chemiluminescent assays

Au-coated capillary based 2

multianalysis

assays

Publikations- och innehållstyp

kon (ämneskategori)

ref (ämneskategori)