On Advances in Asymmetrical Flow Field Flow Fractionation for Biosample Characterization

• Asymmetrical Flow Field Flow Fractionation is a technique with high versatility and is used for size fractionation of polydisperse and widely size distributed samples from various fields of research. Colloidal samples in food systems, formulated biologics from pharmaceutical science, nanoparticles from water treatment systems, molecular distributions in extracted material from wood science, and lipid nano-particles for vaccine production are a few applications where measurements can, have and will helpresearchers to gain better understanding and knowledge of a sample under investigation. In this thesis, two advances in the utilization of AF4, which is the abbreviation for the technique, are presented. These advances can be applied in any of the above mentioned examples. The first paper suggests a novel and more robust calibration methodology for the height of the fractionation channel. The height of the channel is determined to be able to calculate the hydrodynamic size of analytes. The second paper is also a method paper, and a proof-of-concept paper for the direct or on-line coupling between the AF4 channel and the flow-through capillary of synchrotron BioSAXS. The utilization of synchrotron X-rays of high flux was considered necessary for the continuous collection of X-ray scattering from dilute, weakly sattering biologics in constant flow or motion.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

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