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A new animal produc...
A new animal product free defined medium for 2D and 3D culturing of normal and cancer cells to study cell proliferation and migration as well as dose response to chemical treatment
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- Rafnsdóttir, Ólöf Birna (författare)
- University of Iceland,Lund University
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- Kiuru, Anna (författare)
- Lund University,Skåne University Hospital
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- Tebäck, Mattis (författare)
- Lund University
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- Friberg, Nathalie (författare)
- Lund University
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- Revstedt, Philippa (författare)
- Lund University
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- Zhu, Johan (författare)
- Lund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science,Region Skåne
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- Thomasson, Sofia (författare)
- Lund University,Atos Medical AB
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- Czopek, Agnieszka (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Zoofysiologi,Forskargrupper vid Lunds universitet,Functional zoology,Department of Biology,Faculty of Science,Animal Physiology,Lund University Research Groups
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- Malakpour-Permlid, Atena (författare)
- Lund University,Lunds universitet,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Zoofysiologi,Forskargrupper vid Lunds universitet,LTH profilområde: Nanovetenskap och halvledarteknologi,LTH profilområden,Other operations, LTH,Faculty of Engineering, LTH,Functional zoology,Department of Biology,Faculty of Science,Animal Physiology,Lund University Research Groups,LTH Profile Area: Nanoscience and Semiconductor Technology,LTH Profile areas,Faculty of Engineering, LTH,Technical University of Denmark
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- Weber, Tilo (författare)
- German Animal Welfare Federation
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- Oredsson, Stina (författare)
- Lund University,Lunds universitet,Funktionell zoologi,Biologiska institutionen,Naturvetenskapliga fakulteten,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Functional zoology,Department of Biology,Faculty of Science,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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(creator_code:org_t)
- 2023
- 2023
- Engelska 12 s.
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Ingår i: Toxicology Reports. - 2214-7500. ; 10, s. 509-520
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cell culturing methods are increasingly used to reduce and replace the use of live animals in biomedical research and chemical toxicity testing. Although live animals are avoided when using cell culturing methods, they often contain animal-derived components of which one of the most commonly used is foetal bovine serum (FBS). FBS is added to cell culture media among other supplements to support cell attachment/spreading and cell proliferation. The safety, batch-to-batch variation, and ethical problems with FBS are acknowledged and therefore world-wide efforts are ongoing to produce FBS free media. Here, we present the composition of a new defined medium with only human proteins either recombinant or derived from human tissues. This defined medium supports long-term culturing/routine culturing of normal cells and of cancer cells, and can be used for freezing and thawing of cells, i.e. for cell banking. Here, we show for our defined medium, growth curves and dose response curves of cells grown in two and three dimensions, and applications such as cell migration. Cell morphology was studied in real time by phase contrast and phase holographic microscopy time-lapse imaging. The cell lines used are human cancer-associated fibroblasts, keratinocytes, breast cancer JIMT-1 and MDA-MB-231 cells, colon cancer CaCo-2 cells, and pancreatic cancer MiaPaCa-2 cells as well as the mouse L929 cell line. In conclusion, we present the composition of a defined medium without animal-derived products which can be used for routine culturing and in experimental settings for normal cells and for cancer cells, i.e. our defined medium provides a leap towards a universal animal product free cell culture medium.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Animal product free defined medium
- Cancer cells
- Cell migration
- Dose response curves
- Growth curves
- Normal cells
- Routine culturing
- Three dimensions
- Time-lapse imaging
- Two dimensions
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Rafnsdóttir, Ólö ...
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Kiuru, Anna
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Tebäck, Mattis
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Friberg, Nathali ...
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Revstedt, Philip ...
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Zhu, Johan
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visa fler...
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Thomasson, Sofia
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Czopek, Agnieszk ...
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Malakpour-Permli ...
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Weber, Tilo
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Oredsson, Stina
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Toxicology Repor ...
- Av lärosätet
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Lunds universitet