Robotic implementation of a microchip-based protein clean-up and enrichment system for MALDI-TOF MS readout

• A capillary filling microfluidic proteomic sample processing system has been realized in an automated set-up. Chip-integrated solid-phase extraction is performed in a 96-array format followed by sequential capillary action elution into a piezoelectric microdispenser and subsequent transfer to MALDI targets. Samples are eluted and deposited in volumes of 200–300 nl. The robotic system offers calibration to user-defined microextraction arrays and MALDI-target formats at micrometre resolution. Built-in force feedback control ensures a precise and robust microchip docking/handling in three dimensions. An efficient automated washing protocol eliminates analyte carry-over. System throughput ranges typically from 50–100 samples h-1.

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

TEKNIK OCH TEKNOLOGIER  -- Medicinteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Medical Engineering (hsv//eng)

Nyckelord

piezodispensing

capillary force

microfluidics

MALDI

mass spectrometry

microchip

robotic

MALDI-TOF MS

automation

protein analysis

proteomics

solid phase extraction

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