Suppression by neuropeptide Y of capsaicin-sensitive sensory nerve-mediated contraction in guinea-pig airways

• 1. In the present study we have examined whether neuropeptide Y (NPY) interferes with non-adrenergic, non-cholinergic nerve-mediated contractions and relaxations in the guinea-pig airways. In these experiments we have used ring preparations of bronchi and trachea, incubated in the presence of atropine, propranolol and indomethacin (each 1 microM). 2. The contractile response to electrical stimulation of non-adrenergic, non-cholinergic nerve fibres was suppressed by NPY and NPY 13-36 in a concentration-dependent manner, these agents having similar inhibitory potencies. NPY caused a more complete inhibition than the C terminal fragment. 3. NPY affected neither the basal tension nor the substance P-evoked contraction in the bronchi and trachea and did not interfere with nerve-mediated, non-adrenergic relaxation in the trachea. 4. On the basis of these results, it is suggested that NPY may act on the terminals of sensory neurones in the airways to prevent antidromic, excitatory neurotransmission by inhibiting transmitter release.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

Animals

Atropine/pharmacology

Bronchi/drug effects

Capsaicin/pharmacology

Electric Stimulation

Guinea Pigs

In Vitro Techniques

Indomethacin/pharmacology

Male

Muscle Contraction/drug effects

Muscle, Smooth/drug effects

Neurons, Afferent/drug effects

Neuropeptide Y/pharmacology

Propranolol/pharmacology

Substance P/pharmacology

Synaptic Transmission/drug effects

Tetrodotoxin/pharmacology

Trachea/drug effects

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)