Molecular cloning of human prostate specific antigen cDNA

• A lambda gt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library. The cDNA insert of 1415 nucleotides hybridizes specifically to a prostate mRNA species of 1.5 kb. The nucleotide sequence codes for part of a signal peptide, a short propiece and the mature protein of 237 amino acid residues. The Mr for the non-glycosylated protein was 26,089. One potential site for N-linked carbohydrate attachment was identified. The primary structure shows extensive homology with proteases of the kallikrein family.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Medicinal Chemistry (hsv//eng)

Nyckelord

Non-U.S. Gov't

Research Support

Prostate-Specific Antigen

Prostate/immunology

Male

Humans

DNA/*genetics

Molecular

Cloning

Base Sequence

Amino Acid Sequence

Antigens/*genetics

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)