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Modeling Metastatic...
Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor
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- Rafaeva, Maria (author)
- University of Copenhagen
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- Horton, Edward R. (author)
- University of Copenhagen
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- Jensen, Adina R.D. (author)
- University of Copenhagen
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- Madsen, Chris D. (author)
- Lund University,Lunds universitet,Avdelningen för translationell cancerforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancer and matrix remodelling,Forskargrupper vid Lunds universitet,LUCC: Lunds universitets cancercentrum,Övriga starka forskningsmiljöer,Division of Translational Cancer Research,Department of Laboratory Medicine,Faculty of Medicine,Lund University Research Groups,LUCC: Lund University Cancer Centre,Other Strong Research Environments
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- Reuten, Raphael (author)
- University of Copenhagen
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- Willacy, Oliver (author)
- University of Copenhagen
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- Brøchner, Christian B. (author)
- Copenhagen University Hospital
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- Jensen, Thomas H. (author)
- Copenhagen University Hospital
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- Zornhagen, Kamilla Westarp (author)
- University of Copenhagen
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- Crespo, Marina (author)
- University of Copenhagen
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- Grønseth, Dina S. (author)
- University of Copenhagen
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- Nielsen, Sebastian R. (author)
- University of Copenhagen
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- Idorn, Manja (author)
- Herlev Hospital
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- Straten, Per thor (author)
- Herlev Hospital
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- Rohrberg, Kristoffer (author)
- Copenhagen University Hospital
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- Spanggaard, Iben (author)
- Copenhagen University Hospital
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- Højgaard, Martin (author)
- Copenhagen University Hospital
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- Lassen, Ulrik (author)
- Copenhagen University Hospital
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- Erler, Janine T. (author)
- University of Copenhagen
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- Mayorca-Guiliani, Alejandro E. (author)
- University of Copenhagen
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(creator_code:org_t)
- 2021-11-17
- 2022
- English.
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In: Advanced healthcare materials. - : Wiley. - 2192-2640 .- 2192-2659. ; 11:1
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Abstract
Subject headings
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- Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- cancer metastasis
- experimental methods
- extracellular matrix
- specialized bioreactors
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Rafaeva, Maria
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Horton, Edward R ...
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Jensen, Adina R. ...
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Madsen, Chris D.
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Reuten, Raphael
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Willacy, Oliver
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show more...
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Brøchner, Christ ...
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Jensen, Thomas H ...
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Zornhagen, Kamil ...
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Crespo, Marina
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Grønseth, Dina S ...
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Nielsen, Sebasti ...
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Idorn, Manja
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Straten, Per tho ...
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Rohrberg, Kristo ...
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Spanggaard, Iben
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Højgaard, Martin
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Lassen, Ulrik
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Erler, Janine T.
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Mayorca-Guiliani ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Advanced healthc ...
- By the university
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Lund University