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Investigation of th...
Investigation of the enzyme Bacillus agaradhaerens Cel 5a as an analytical tool in mass spectral characterisation of methylcelluloses
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- Cohen, Arieh (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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- Nilsson, Carina (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Schagerlöf, Herje (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Tjerneld, Folke (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Gorton, Lo (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centrum för analys och syntes,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Centre for Analysis and Synthesis,Faculty of Engineering, LTH
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
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Ingår i: Analytica Chimica Acta. - : Elsevier BV. - 1873-4324 .- 0003-2670. ; 561:1-2, s. 16-24
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Two methylcelluloses (MC) were investigated by partial hydrolysis followed by direct infusion electrospray ionisation mass spectrometry (ESI-MS). Partial hydrolysis was achieved either enzymatically or by acid hydrolysis. Enzyme hydrolysis was performed using the endoglucanase Bacillus agaradhaerens Cel 5a. Three different hydrolysis buffer solutions were investigated in order to deter-mine which was most compatible with the subsequent MS analysis. ESI-MS experiments showed that when using enzymatic hydrolysis as an analytical tool for characterisation of modified cellulose one has to be watchful for the investigated polymer to have a sufficient amount of available cleavage sites in order to ensure that the original ends of the polymer do not influence the results. MS2 experiments confirmed that the detected products originated from methylcellulose and provided structural information on the investigated products. MS data proved that B. agaradhaerens Cel 5a is able to cleave beta 1 -> 4 glycosidic linkages adjacent to a permethylated glucosyl unit. MS2 data showed that methylation on the C-2 and C-6 hydroxyl groups of the glucosyl unit on the non-reducing side of the cleavage site hindered the enzyme. (c) 2006 Elsevier B.V. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Nyckelord
- Cel 5a
- Bacillus agaradhaerens
- ESI-MS
- mass spectrometry
- methylcellulose
- MC
- cellulase
- modification pattern
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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