Sökning: id:"swepub:oai:lup.lub.lu.se:5e5b67bd-60b9-42a5-89e8-69d258e164cf" >
Adsorption of human...
Adsorption of human carbonic anhydrase II onto silicon oxides surfaces : The effects of truncation in the N-terminal region
-
- Billsten, P (författare)
- Linköping University
-
- Wahlgren, M (författare)
- Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
-
- Elwing, H (författare)
- University of Gothenburg
-
visa fler...
-
Lindman, B. (redaktör/utgivare)
-
Ninham, B. W. (redaktör/utgivare)
-
visa färre...
-
(creator_code:org_t)
- 1998
- 1998
- Engelska 5 s.
-
Ingår i: The Colloid Science of Lipids New Paradigms for Self-Assembly in Science and Technology. - 1435-1536 .- 0340-255X. - 9783798511125 - 9783798516557 ; 108, s. 161-165
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The adsorption of human carbonic anhydrase II pseudo-wild type (HCAIIpwt) and an N-terminally truncated version thereof onto silica surfaces were studied. The amount adsorbed and the adsorption kinetics were measured using in situ ellipsometry. A substantial difference was seen between the two proteins. The adsorbed amount of the truncated version (2.53 mg/m2) indicates an end-on orientation, while the HCAIIpwt seems to adsorb side-on (1.84 mg/m2). It is suggested that the orientation effects arise from the truncation. The truncation is known to unfold the two most N-terminal helical segments, which could inhibit adsorption with the N-terminal region facing the surface, due to steric repulsion.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Annan teknik -- Livsmedelsteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies -- Food Engineering (hsv//eng)
Nyckelord
- Protein adsorption
- Ellipsometry
- Carbonic Anhydrases
- Mutant proteins
- Solid Surfaces
Publikations- och innehållstyp
- kon (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas