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Cross-linked enzyme...
Cross-linked enzyme aggregates of pig liver esterase evaluated in kinetic resolution of racemic clopidogrel
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- Gaber, Yasser (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Ismail, Mohamed (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Science Alert, 2017
- 2017
- Engelska 7 s.
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Ingår i: Biotechnology (Faisalabad). - : Science Alert. - 1682-296X. ; 16:3, s. 123-129
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Background and Objective: Immobilization of enzymes as cross-linked aggregates is one of the cheapest, simplest and effective techniques for improving their stability and reusability and even avoiding contamination of the product with the catalyst. Clopidogrel is a widely used antiplatelet drug, only Sisomer is the biologically active enantiomer produced by resolution of the racemic compound. In the current study, cross-linked aggregates of pig liver esterase were prepared and evaluated for kinetic resolution of racemic clopidogrel. Materials and Methods: Cross-linked Enzyme Aggregates (CLEA) of the commercially available crude pig liver esterase cPLE were prepared using glutaraldehyde at concentrations of 12.5-125 mM as crosslinker either in presence or absence of Bovine serum albumin (BSA). cPLE-CLEA was used for kinetic resolution of racemic clopidogrel and compared to the performance of soluble cPLE. Light microscopy and scanning electron microscopy SEM were used to examine cPLE-CLEA. Results: Soluble cPLE showed ability to resolve racemic clopidogrel at enantioselectivity (E) of 9.2. The resolution of clopidogrel was found to be optimal at 30°C. The cPLE-CLEA preparations showed reduced enzymatic activity. The kinetic resolution experiments showed also lower E values (E = 1.3-4.5) compared to soluble cPLE. Microscopical examination of cPLE-CLEA showed wide size variation and SEM revealed the shape of cPLE-CLEA before and after use in the kinetic resolution experiments. Conclusion: Crude PLE was able to resolve racemic clopidogrel, the effects of different temperatures were studied and the highest E value recorded was 9.2 at 30°C. Increasing concentrations of glutaraldehyde as a cross-linker adversely affected PLE activity. The cPLE-CLEA showed lower enantioselectivity compared to the free cPLE.
Nyckelord
- CLEA
- Clopidogrel
- Enantioselectivity
- Esterase
- Glutaraldehyde
- Kinetic resolution
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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