The occurrence of neuropeptides at different stages of DBM-induced heterotopic bone formation

• In developing heterotopic bone in the rat, induced by allogeneic bone matrix, we immunohistochemically detected nerves containing substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP) and tyrosine hydroxylase (TH). After 10 days they were dicernible amidst differentiating chondroblastoid cells in fibrous tissue around and within the implants. Over the next 3 weeks, the nerves increased in number and gradually attained a shape and distribution resembling normal osseal nerves; varicose fibres frequently occurred in periosteum-like fibrous tissue and bone marrow adjacent to newly formed bone. At 8 weeks, NPY-fibres increased, particularly in the marrow and this abundance of NPY fibres remained at 16 weeks. VIP-immunoreactive fibres were only observed in the surrounding periosteum-like fibrous tissue 4-6 weeks after implantation. These observations, in combination with recent findings of receptors to neuropeptides on bone cells, suggest a neurogenic influence on physiological processes in bone tissue.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Ortopedi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Orthopaedics (hsv//eng)

Nyckelord

Animals

Bone Development/physiology

Bone Matrix/transplantation

Bone and Bones/innervation

Calcification, Physiologic

Calcitonin Gene-Related Peptide/metabolism

Fluorescent Antibody Technique

Male

Neuropeptide Y/metabolism

Neuropeptides/metabolism

Rats

Rats, Inbred Strains

Substance P/metabolism

Transplantation, Heterotopic

Tyrosine 3-Monooxygenase/metabolism

Vasoactive Intestinal Peptide/metabolism

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)