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Phase-contrast enha...
Phase-contrast enhanced synchrotron micro-tomography of human meniscus tissue
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- Einarsson, E (författare)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Medicinsk strålningsfysik, Malmö,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Department of Translational Medicine,Faculty of Medicine,Medical Radiation Physics, Malmö,Lund University Research Groups
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- Pierantoni, M (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH
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- Novak, V (författare)
- Paul Scherrer Institute
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- Svensson, J (författare)
- Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Medicinsk strålningsfysik, Malmö,Forskargrupper vid Lunds universitet,Lund University Bioimaging Center,Department of Translational Medicine,Faculty of Medicine,Medical Radiation Physics, Malmö,Lund University Research Groups
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- Isaksson, H (författare)
- Lund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH
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- Englund, M (författare)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Lund University Bioimaging Center,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,CPUP - barnortopedisk forskningsgrupp,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups,CPUP - Children's Orthopedics Group
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- Engelska 12 s.
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Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 30:9, s. 1222-1233
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- OBJECTIVE: To investigate the feasibility of synchrotron radiation-based phase contrast enhanced micro-computed tomography (SR-PhC-μCT) for imaging of human meniscus. Quantitative parameters related to fiber orientation and crimping were evaluated as potential markers of tissue degeneration.DESIGN: Human meniscus specimens from 10 deceased donors were prepared using different preparation schemes: fresh frozen and thawed before imaging or fixed and paraffin-embedded. The samples were imaged using SR-PhC-μCT with an isotropic voxel size of 1.625 μm. Image quality was evaluated by visual inspection and spatial resolution. Fiber voxels were defined using a grey level threshold and a structure tensor analysis was applied to estimate collagen fiber orientation. The area at half maximum (FAHM) was calculated from angle histograms to quantify orientation distribution. Crimping period was calculated from the power spectrum of image profiles of crimped fibers. Parameters were compared to degenerative stage as evaluated by Pauli histopathological scoring.RESULTS: Image quality was similar between frozen and embedded samples and spatial resolutions ranged from 5.1 to 5.8 μm. Fiber structure, including crimping, was clearly visible in the images. Fibers appeared to be less organized closer to the tip of the meniscus. Fiber density might decrease slightly with degeneration. FAHM and crimping period did not show any clear association with histopathological scoring.CONCLUSION: SR-PhC-μCT is a feasible technique for high-resolution 3D imaging of fresh frozen meniscus tissue. Further work is needed to establish quantitative parameters that relate to tissue degeneration, but this imaging technique is promising for future studies of meniscus structure and biomechanical response.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- Humans
- Meniscus
- Microscopy, Phase-Contrast
- Synchrotrons
- Tomography
- X-Ray Microtomography
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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