Search: id:"swepub:oai:lup.lub.lu.se:725e1f56-b45f-431d-ab4d-856628d6af72" >
Gene expression ana...
Gene expression analysis suggests that EBF-1 and PPAR gamma 2 induce adipogenesis of NIH-3T3 cells with similar efficiency and kinetics
-
- Akerblad, P (author)
- AstraZeneca Research
-
- Månsson, Robert (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy
-
- Lagergren, Anna (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy
-
show more...
-
- Westerlund, S (author)
- AstraZeneca Research
-
- Basta, B (author)
- AstraZeneca Research
-
- Lind, U (author)
- AstraZeneca Research
-
- Thelin, A (author)
- AstraZeneca Research
-
- Gisler, Ramiro (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy
-
- Liberg, David (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy
-
- Nelander, S (author)
- Chalmers Technical University
-
- Bamberg, K (author)
- AstraZeneca Research
-
- Sigvardsson, Mikael (author)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Lund Strategic Research Center for Stem Cell Biology and Cell Therapy
-
show less...
-
(creator_code:org_t)
- American Physiological Society, 2005
- 2005
- English.
-
In: Physiological Genomics. - : American Physiological Society. - 1094-8341 .- 1531-2267. ; 23:2, s. 206-216
- Related links:
-
http://dx.doi.org/10... (free)
-
show more...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Differentiation of multipotent mesenchymal stem cells into lipid-accumulating adipocytes is a physiological process induced by transcription factors in combination with hormonal stimulation. We have used Affymetrix microarrays to compare the adipogenic differentiation pathways of NIH-3T3 fibroblasts induced to undergo in vitro differentiation by ectopic expression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma 2. These experiments revealed that commitment to the adipogenic pathway in the NIH-3T3 cells was not reflected in gene expression until 4 days after induction of differentiation. Furthermore, gene expression patterns at the earlier time points after stimulation indicated that EBF-1 and PPAR gamma 2 induced different sets of genes, while the similarities increased upon differentiation, and that several genes linked to adipocyte differentiation were also transiently induced in the vector-transduced cells. These data suggest that the initial activation of genes associated with adipocyte development is independent of commitment to the adipogenic pathway and that EBF-1 and PPAR gamma 2 induce adipocyte differentiation with comparable kinetics and efficiency.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- early B cell factor
- peroxisome proliferator-activated
- adipogenesis
- receptor-gamma 2
- adipogenesis; early B cell factor; peroxisome proliferator-activated receptor-gamma 2
- TECHNOLOGY
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
To the university's database
- By the author/editor
-
Akerblad, P
-
Månsson, Robert
-
Lagergren, Anna
-
Westerlund, S
-
Basta, B
-
Lind, U
-
show more...
-
Thelin, A
-
Gisler, Ramiro
-
Liberg, David
-
Nelander, S
-
Bamberg, K
-
Sigvardsson, Mik ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Physiological Ge ...
- By the university
-
Lund University
-
Linköping University