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Separation of 100-k...
Separation of 100-kilobase DNA molecules in 10 seconds
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- Bakajin, O. (författare)
- Princeton University,Lawrence Livermore National Laboratory
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- Duke, T. A J (författare)
- University of Cambridge
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- Tegenfeldt, J. (författare)
- Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Princeton University
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- Chou, C. F. (författare)
- Princeton University
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- Chan, S. S. (författare)
- Lund University,Lunds universitet,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Physics,Departments at LTH,Faculty of Engineering, LTH,Princeton University
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- Austin, R. H. (författare)
- Princeton University
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- Cox, E. C. (författare)
- Princeton University
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(creator_code:org_t)
- 2001-11-02
- 2001
- Engelska 4 s.
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 73:24, s. 6053-6056
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.
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