Oxygen consumption of the aerobically-perfused cardioplegic donor heart at different temperatures

• Background: The aim of this study was to investigate the oxygen consumption of explanted aerobically-perfused cardioplegic porcine hearts at different temperatures. Material/Methods: Explanted hearts from 30 pigs weighing 50 kg were randomized into 5 groups. The hearts received continuous antegrade perfusion within a temperature-controlled sealed system. The perfusate consisted of an albumincontaining hyperoncotic cardioplegic nutrition solution with erythrocytes to a hematocrit of 10%. Five temperatures were studied: 37, 30, 22, 15, and 8°C. When the erythrocytes in the perfusate were fully saturated, the oxygenator was excluded from the circuit and blood gases were analyzed periodically until the erythrocytes had desaturated to less than 20%. Between 80% and 60% saturation the desaturation curves were linear in all groups and the oxygen consumption was calculated from this part of the curves. Results: The weight of the hearts was 208±4 g (mean ±SEM, n=30). The oxygen consumption in mL/min/100 g heart tissue was (mean ±SEM, n=6 in each group) 37°C: 1.10±0.04; 30°C: 0.58±0.02; 22°C: 0.33±0.01; 15°C: 0.21±0.01; and 8°C: 0.16±0.02. Conclusions: The oxygen consumption of the cardioplegic perfused pig heart at normothermia was 1.1 mL/min/100 g and was reduced by 85% to 0.16 mL/min/100 g at 8°C.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kardiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)

Nyckelord

Heart

Metabolism

Organ preservation

Oxygen consumption

Perfusion

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