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Dose-dependent effe...
Dose-dependent effects of acetaminophen and ibuprofen on mitochondrial respiration of human platelets
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- Beţiu, Alina Maria (författare)
- Victor Babes University of Medicine and Pharmacy
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- Lighezan, Rodica (författare)
- Victor Babes University of Medicine and Pharmacy
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- Avram, Vlad Florian (författare)
- Victor Babes University of Medicine and Pharmacy
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- Muntean, Danina Mirela (författare)
- Victor Babes University of Medicine and Pharmacy
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- Elmér, Eskil (författare)
- Lund University,Lunds universitet,Klinisk neurofysiologi,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Mitokondriell Medicin,Forskargrupper vid Lunds universitet,Clinical Neurophysiology,Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine,Mitochondrial Medicine,Lund University Research Groups,Abliva AB
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- Petrescu, Lucian (författare)
- Victor Babes University of Medicine and Pharmacy
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(creator_code:org_t)
- Engelska.
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Ingår i: Molecular and Cellular Biochemistry. - 0300-8177.
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Acetaminophen and ibuprofen are widely used over-the-counter medications to reduce fever, pain, and inflammation. Although both drugs are safe in therapeutic concentrations, self-medication is practiced by millions of aged patients with comorbidities that decrease drug metabolism and/or excretion, thus raising the risk of overdosage. Mitochondrial dysfunction has emerged as an important pathomechanism underlying the organ toxicity of both drugs. Assessment of mitochondrial oxygen consumption in peripheral blood cells is a novel research field Cu several applications, including characterization of drug toxicity. The present study, conducted in human platelets isolated from blood donor-derived buffy coat, was aimed at assessing the acute, concentration-dependent effects of each drug on mitochondrial respiration. Using the high-resolution respirometry technique, a concentration-dependent decrease of oxygen consumption in both intact and permeabilized platelets was found for either drug, mainly by inhibiting complex I-supported active respiration. Moreover, ibuprofen significantly decreased the maximal capacity of the electron transport system already from the lowest concentration. In conclusion, platelets from healthy donors represents a population of cells easily available, which can be routinely used in studies assessing mitochondrial drug toxicity. Whether these results can be recapitulated in patients treated with these medications is worth further investigation as potential peripheral biomarker of drug overdose.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Acetaminophen
- Buffy coat
- Human platelets
- Ibuprofen
- Mitochondria
- Respiration
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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