Sökning: id:"swepub:oai:lup.lub.lu.se:8234947e-c081-4209-854f-abccf8287343" >
Single-molecule den...
Single-molecule denaturation mapping of DNA in nanofluidic channels
-
Reisner, Walter (författare)
-
Larsen, Niels B. (författare)
-
Silahtaroglu, Asli (författare)
-
visa fler...
-
Kristensen, Anders (författare)
-
Tommerup, Niels (författare)
-
- Tegenfeldt, Jonas (författare)
- Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
-
Flyvbjerg, Henrik (författare)
-
visa färre...
-
(creator_code:org_t)
- 2010-07-07
- 2010
- Engelska.
-
Ingår i: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 107:30, s. 13294-13299
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://www.pnas.org...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Here we explore the potential power of denaturation mapping as a single-molecule technique. By partially denaturing YOYO (R)-1-labeled DNA in nanofluidic channels with a combination of formamide and local heating, we obtain a sequence-dependent "barcode" corresponding to a series of local dips and peaks in the intensity trace along the extended molecule. We demonstrate that this structure arises from the physics of local denaturation: statistical mechanical calculations of sequence-dependent melting probability can predict the barcode to be observed experimentally for a given sequence. Consequently, the technique is sensitive to sequence variation without requiring enzymatic labeling or a restriction step. This technique may serve as the basis for a new mapping technology ideally suited for investigating the long-range structure of entire genomes extracted from single cells.
Ämnesord
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
Nyckelord
- DNA optical mapping
- DNA barcoding
- DNA denaturation
- nanochannel
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas