Immunocapillarymigration with enzyme-labeled antibodies : rapid quantification of C-reactive protein in human plasma

• Various procedures to improve the sensitivity and precision of antigen quantitation by immunocapillarymigration are investigated. The best results are obtained when using porous strips of cellulose acetate with covalently attached antibodies and when enzyme-labeled antibodies are used to expose the antigen-covered areas of the strips. Such a system has a sensitivity of 0.15 mg/liter and a precision of 7%. It allows a rapid quantitation of human C-reactive protein without the use of laboratory instrumentation.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

C-Reactive Protein/analysis

Capillary Action

Cellulose/analogs & derivatives

Drug Stability

Humans

Immunoelectrophoresis

Immunoenzyme Techniques/standards

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)