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Asymmetrical flow f...
Asymmetrical flow field-flow fractionation enables the characterization of molecular and supramolecular properties of cereal beta-glucan dispersions
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- Håkansson, Andreas (author)
- Lund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Ulmius, Matilda (author)
- Lund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Faculty of Engineering, LTH
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- Nilsson, Lars (author)
- Lund University,Lunds universitet,Livsmedelsteknik,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Food Technology, Engineering and Nutrition,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Carbohydrate Polymers. - : Elsevier BV. - 0144-8617. ; 87:1, s. 518-523
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Abstract
Subject headings
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- In this paper we study the properties of molecular and supra molecular species in cereal beta-glucan solutions/dispersions by the utilization of asymmetrical flow field-flow fractionation coupled to multi-angle light scattering and refractive index (AsFlFFF-MALS-RI) detectors. The samples were purified barley and oat beta-glucans which were dissolved in aqueous solution using either mild conditions or more harsh treatments with alkali. Dissolution in 0.5 M NaOH was not sufficient to eliminate aggregated structures in barley beta-glucan. The results in this paper show how distinction can possibly be made between molecular and supra molecular species using scaling approaches and conformational parameters obtained from AsFlFFF-MALS-RI over the entire size distribution. Small species in the barley beta-glucan samples display properties ranging from elongated conformation to random coil conformation. Aggregates have low apparent densities and a swollen micro gel structure. Oat beta-glucan displays no properties that can be attributed to a molecularly dissolved beta-glucan showing that dissolution was incomplete. The aggregate properties analyzed were similar between oat and barley beta-glucan.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Annan teknik -- Livsmedelsteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Other Engineering and Technologies -- Food Engineering (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Beta-glucan Asymmetrical flow field-flow fractionation AF4 Oat Barley
Publication and Content Type
- art (subject category)
- ref (subject category)
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