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Screening for weak ...
Screening for weak monoclonal antibodies in hybridoma technology
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- Leickt, L (författare)
- University of Kalmar
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- Grubb, A (författare)
- Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cystatin C, njursjukdom, amyloidos och antibiotika,Forskargrupper vid Lunds universitet,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Cystatin C, renal disease, amyloidosis and antibiotics,Lund University Research Groups
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- Ohlson, Sten (författare)
- University of Kalmar
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(creator_code:org_t)
- 1-6
- 1998
- 1998
- Engelska.
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Ingår i: Journal of Molecular Recognition : Special Issue: Proceedings of the 12th International Symposium on Affinity Interactions - Special Issue: Proceedings of the 12th International Symposium on Affinity Interactions. - 0952-3499. ; 11, s. 114-116
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Abstract
Ämnesord
Stäng
- As the interest in weak-affinity antibodies has been widened by their introduction to various analytical techniques such as HPLC, capillary electrophoresis and biosensors, there has been a need for new screening/monitoring methods. In this study, weak-affinity chromatography was adopted to screen/monitor directly for monoclonal antibodies in ascites. Monoclonal antibodies against a carbohydrate antigen (maltohexaose) were used to evaluate this approach. In short, malthohexaose was immobilized on an HPLC support in such a configuration to allow, during HPLC, retardation of weak monoclonal antibodies. Based on the retention, the affinity or the avidity, as determined by the presence of multiple binding of the monoclonal antibody towards antigen, can be estimated. In this way it is possible to select clones of hybridomas that produce desired weak monoclonal antibodies. Adjustments in temperature (10-20 degrees C) were used to moderate the retention and hence affinity of the weak monoclonal antibodies during chromatography.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Animals
- Antibodies, Monoclonal/isolation & purification
- Antibody Affinity
- Ascites/immunology
- Carbohydrate Sequence
- Chromatography, Affinity
- Chromatography, High Pressure Liquid
- Hybridomas/immunology
- Mice
- Molecular Sequence Data
- Oligosaccharides/chemistry
Publikations- och innehållstyp
- kon (ämneskategori)
- ref (ämneskategori)
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