Sökning: id:"swepub:oai:lup.lub.lu.se:8f24d864-3b63-406c-86ff-1fb9642d8f82" >
Orientational Ambig...
Orientational Ambiguity in Septin Coiled Coils and its Structural Basis
-
- Leonardo, Diego A. (författare)
- University of São Paulo
-
- Cavini, Italo A. (författare)
- University of São Paulo
-
- Sala, Fernanda A. (författare)
- University of São Paulo
-
visa fler...
-
- Mendonça, Deborah C. (författare)
- University of São Paulo
-
- Rosa, Higor V.D. (författare)
- University of São Paulo
-
- Kumagai, Patricia S. (författare)
- University of São Paulo
-
- Crusca, Edson (författare)
- University of São Paulo
-
- Valadares, Napoleão F. (författare)
- University of Brasilia
-
- Marques, Ivo A. (författare)
- University of São Paulo
-
- Brandão-Neto, José (författare)
- Diamond Light Source
-
- Munte, Claudia E. (författare)
- University of Regensburg
-
- Kalbitzer, Hans R. (författare)
- University of Regensburg
-
- Soler, Nicolas (författare)
- CSIC - Institute of Molecular Biology of Barcelona (IBMB)
-
- Usón, Isabel (författare)
- CSIC - Institute of Molecular Biology of Barcelona (IBMB),Catalan Institution for Research and Advanced Studies
-
- André, Ingemar (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
- Araujo, Ana P.U. (författare)
- University of São Paulo
-
- D'Muniz Pereira, Humberto (författare)
- University of São Paulo
-
- Garratt, Richard C. (författare)
- University of São Paulo
-
visa färre...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- Engelska.
-
Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836. ; 433:9
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Septins are an example of subtle molecular recognition whereby different paralogues must correctly assemble into functional filaments important for essential cellular events such as cytokinesis. Most possess C-terminal domains capable of forming coiled coils which are believed to be involved in filament formation and bundling. Here, we report an integrated structural approach which aims to unravel their architectural diversity and in so doing provide direct structural information for the coiled-coil regions of five human septins. Unexpectedly, we encounter dimeric structures presenting both parallel and antiparallel arrangements which are in consonance with molecular modelling suggesting that both are energetically accessible. These sequences therefore code for two metastable states of different orientations which employ different but overlapping interfaces. The antiparallel structures present a mixed coiled-coil interface, one side of which is dominated by a continuous chain of core hydrophilic residues. This unusual type of coiled coil could be used to expand the toolkit currently available to the protein engineer for the design of previously unforeseen coiled-coil based assemblies. Within a physiological context, our data provide the first atomic details related to the assumption that the parallel orientation is likely formed between septin monomers from the same filament whilst antiparallelism may participate in the widely described interfilament cross bridges necessary for higher order structures and thereby septin function.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- coiled coil
- crystal structures
- mixed hydrophobic/hydrophilic interface
- protein filament
- septins
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Leonardo, Diego ...
-
Cavini, Italo A.
-
Sala, Fernanda A ...
-
Mendonça, Debora ...
-
Rosa, Higor V.D.
-
Kumagai, Patrici ...
-
visa fler...
-
Crusca, Edson
-
Valadares, Napol ...
-
Marques, Ivo A.
-
Brandão-Neto, Jo ...
-
Munte, Claudia E ...
-
Kalbitzer, Hans ...
-
Soler, Nicolas
-
Usón, Isabel
-
André, Ingemar
-
Araujo, Ana P.U.
-
D'Muniz Pereira, ...
-
Garratt, Richard ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Journal of Molec ...
- Av lärosätet
-
Lunds universitet