Demonstration And Identification Of Cytotoxic Antibodies And Antibodies Blocking The Cell-Mediated Antitumor Immunity Against Adenovirus Type 12 Induced Tumors

• Hyperimmune mouse antisera against syngeneic adenovirus type 12 induced tumors and their 7 S and 19 S fractions of immunoglobulins obtained after combined diethylaminoethyl cellulose and Sephadex G-200 separation were tested against a rat adenovirus type 12 induced tumor by 51 Cr release technique. The same sera and their fractions were investigated with the colony inhibition technique for ability to abrogate the inhibitory effect of mouse lymph node cells specifically immunized against syngeneic adenovirus type 12 induced tumors on rat adenovirus 12 target tumor cells. It was found that (a) hyperimmune antisera from mice which had received many immunization doses were cytotoxic to rat adenovirus 12 induced tumor cells at 37° in the presence of complement; (b) the same hyperimmune antisera abrogated specifically the inhibitory effect of specifically immune mouse lymph node cells on rat adenovirus 12 tumor cells; (c) the cytotoxic activity of the hyperimmune antisera at 37° in the presence of complement was found in 19 S fractions of γ-globulins; and (d) the serum factors causing abrogation of immune lymph node cells in vitro were found in 7 S fractions of immunoglobulins.

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MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

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