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Growth kinetics and...
Growth kinetics and Pho84 phosphate transporter activity of Saccharomyces cerevisiae under phosphate-limited conditions
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Shokrollahzadeh, Soheila (författare)
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Bonakdarpour, Babak (författare)
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Vahabzadeh, Farzaneh (författare)
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- Sanati, Mehri (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 2006-11-16
- 2007
- Engelska.
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Ingår i: Journal of Industrial Microbiology & Biotechnology. - : Oxford University Press (OUP). - 1367-5435 .- 1476-5535. ; 34:1, s. 17-25
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The effect of phosphate (P ( i )) concentration on the growth behavior of Saccharomyces cerevisiae strain CEN.PK113-5D in phosphate-limited batch and chemostat cultures was studied. The range of dilution rates used in the present study was 0.08-0.45 h(-1). The batch growth of yeast cells followed Monod relationship, but growth of the cells in phosphate-limited chemostat showed change in growth kinetics with increasing dilution rates. The difference in growth kinetics of the yeast cells in phosphate-limited chemostat for dilution rates below and above approximately 0.2 h(-1) has been discussed in terms of the batch growth kinetic data and the change in the metabolic activity of the yeast cells. Immunological detection of a C-terminally myc epitope-tagged Pho84 fusion protein indicated derepressive expression of the Pho84 high-affinity P ( i ) transporter in the entire range of dilution rates employed in this study. Phosphate transport activity mediated by Pho84 transporter was highest at very low dilution rates, i.e. 0.08-0.1 h(-1), corresponding to conditions in which the amount of synthesized Pho84 was at its maximum.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Maskinteknik -- Produktionsteknik, arbetsvetenskap och ergonomi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Mechanical Engineering -- Production Engineering, Human Work Science and Ergonomics (hsv//eng)
Nyckelord
- phosphate
- Pho84 transporter
- growth kinetics
- saccharomyces cerevisiae
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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